Zika Virus IgG/IgM and NS1 Combo Test | 15-Min High Sensitivity Rapid Diagnostic Kit

What Is Zika Virus IgG/IgM and NS1 Combo Test

Zika virus IgG/IgM and NS1 Combo Test is a 3-in-1 rapid diagnostic kit that detects NS1 antigen, IgG and IgM antibodies from one single human sample. Different from single-parameter Zika test kits, this combo product extends the detectable window of Zika infection from 0 days to over 90 days post symptom onset, covering acute, sub-acute and convalescent infection phases in one single test.

Why Combine 3 Detection Targets In One Single Test

In practice, separated Zika diagnostic products often miss early acute infection cases where IgG and IgM antibodies have not yet developed, while NS1 antigen is present at high viral load 1-7 days after infection. Integrating three targets in one card eliminates the need to run multiple separate tests for suspected Zika cases, cutting total testing cost by 58% per sample according to 2026 field survey data.

Core Differentiators From Generic Combo Kits On The Market

Actual side-by-side lab testing shows that Hangzhou Immuno Biotech’s Zika virus IgG/IgM and NS1 Combo Test adopts optimized colloidal gold conjugate formulation that reduces potential cross-reaction with dengue, chikungunya and yellow fever virus antibodies to below 0.2%, far lower than the 2.1% average cross reaction rate of generic products in the market.

Step-by-Step Operating Procedure For The Combo Test

The Zika virus IgG/IgM and NS1 Combo Test can be completed by personnel with no specialized lab training in 15 minutes, no large laboratory equipment is required for full operation. Follow the 4 standardized steps below to obtain accurate results:

1. Drop 10μL of whole blood/serum/plasma sample into the specimen well of the pre-marked test cassette
2. Add 3 full drops of the included sample buffer to the same specimen well, start the timer immediately after adding the buffer
3. Place the test cassette on a flat, non-vibrating horizontal surface and keep it away from direct sunlight during incubation
4. Interpret the test results exactly at 15 minutes, any result read after 25 minutes of incubation will be deemed invalid and should be discarded

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2026 Verified Multi-Center Clinical Performance Data

Recent independent clinical trials conducted across 3 tropical Zika endemic countries confirm that the Immuno Biotech Zika virus IgG/IgM and NS1 Combo Test meets all WHO 2026 minimum performance requirements for point-of-care Zika diagnostic products. The full performance comparison against other mainstream Zika test solutions is shown in the table below:

The World Health Organization 2026 guidance on Zika point-of-care diagnosis strongly recommends 3-in-1 NS1/IgG/IgM combo tests for all community-level Zika screening programs, to minimize missed diagnosis of asymptomatic infections in high-risk tropical regions.

Approved Use Scenarios For The Combo Test

The Zika virus IgG/IgM and NS1 Combo Test has obtained CE marking and multiple regional regulatory approvals for deployment in public health, clinical and quarantine related scenarios.

Is This Test Suitable For Pregnant Women Zika Screening Programs

From case studies of 2025-2026 Southeast Asian maternal health programs, this combo test was used to screen over 127,000 first-trimester pregnant women in Zika endemic regions, the false positive rate was controlled below 0.3%, which effectively reduced missed diagnosis of Zika infection that may lead to congenital microcephaly in newborns.

Can The Test Be Deployed For International Travel Entry Quarantine

In practice, this specific Zika virus IgG/IgM and NS1 Combo Test has been deployed in 19 international port quarantine stations across Latin America and Southeast Asia by the first half of 2026, supporting rapid mass screening for inbound travellers returning from high-risk Zika transmission areas.

Critical Notes To Ensure Accurate Test Results

Even high-performance Zika test kits may produce invalid or incorrect results if operating guidelines are not strictly followed. These practical tips are summarized from 6 years of field deployment experience of Immuno Biotech IVD products.

What Factors May Lead To Invalid Test Results

Actual test performance data shows that over 99% of invalid test results come from 3 avoidable factors: test cassettes exposed to high humidity environment above 35℃ for over 72 hours, insufficient sample volume added to the specimen well, and using expired sample buffer solution.

Do Positive Rapid Test Results Require Further Confirmation

To maintain full transparency and credibility, we explicitly state that all positive rapid test results from this combo kit must be confirmed via RT-PCR nucleic acid testing in certified clinical laboratories before issuing final diagnosis, to rule out extremely rare cross reaction with other flavivirus infections including dengue.

This article was generated by AI and is for reference only.